| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234682 |
| Chromosome: | chromosome 17 |
| Location: | 2947415 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCTAAGCCAACAAAAGCGAGTGTCA |
| Internal bar code: | GCTCCTTGGGGTGCCCTGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1218 |
| LEAP-Seq percent confirming: | 54.2724 |
| LEAP-Seq n confirming: | 3036 |
| LEAP-Seq n nonconfirming: | 2558 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGAAAGACAGCCTGAGT |
| Suggested primer 2: | CTGACGACTCCCTCACCTTC |