Insertion junction: LMJ.RY0402.234691_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.234691
Chromosome:chromosome 7
Location:2380251
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328500 ARS17 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGTTGCAACCCCGCTCGCCCCCGTACT
Internal bar code:TAGTCTGAGCAACTTCCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:660
LEAP-Seq percent confirming:99.8074
LEAP-Seq n confirming:2073
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGTTTCAAACCCTCAGA
Suggested primer 2:AGACGTGATACGGGACAAGG