| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234701 |
| Chromosome: | chromosome 3 |
| Location: | 5439038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185150 | (1 of 11) IPR023109 - Integrase/recombinase, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGTTGGGGGTGGGAACTGAACTCAAG |
| Internal bar code: | CAAAGCGTATGTACTGGTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 1550 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGGACAAGGTGAGCGA |
| Suggested primer 2: | GGAGTGTCGCTCCTGCTATC |