| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234713 |
| Chromosome: | chromosome 10 |
| Location: | 3426999 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444600 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCACTTGCCCAGCAGTCCCCCAAGGCC |
| Internal bar code: | AGGGTTTCCAACTTGCAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 99.4793 |
| LEAP-Seq n confirming: | 4967 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACTGGACATACAGCAGC |
| Suggested primer 2: | CGCCCCATTCTTCATGTACT |