| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234713 |
| Chromosome: | chromosome 10 |
| Location: | 3427001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444600 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGCCCTCTAAGGCCAAAACCGCACAAC |
| Internal bar code: | GGCTGTCCTGCGAACCAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 98.0857 |
| LEAP-Seq n confirming: | 3433 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACTGGACATACAGCAGC |
| Suggested primer 2: | CGCCCCATTCTTCATGTACT |