| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234714 |
| Chromosome: | chromosome 17 |
| Location: | 5102183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734961 | (1 of 1) PTHR15239:SF6 - NUCLEAR EXPORT MEDIATOR FACTOR NEMF | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCATCCTTAAAGAAGTAGGGAAATTCTT |
| Internal bar code: | CGACATGTGTGAAGAGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 71.0207 |
| LEAP-Seq n confirming: | 4627 |
| LEAP-Seq n nonconfirming: | 1888 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCCAGGAACAGTAGC |
| Suggested primer 2: | TGCCATATGTGAAGGCATGT |