| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234758 |
| Chromosome: | chromosome 16 |
| Location: | 4961208 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685400 | TIC32-3,SDR26 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.105//1.1.1.300 - All-trans-retinol dehydrogenase (NAD(+)) // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTGCGGACGCCGCAGGTACTACTCTCG |
| Internal bar code: | ATTTGACAGTAACTCAGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 99.5768 |
| LEAP-Seq n confirming: | 13882 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCCAATGCTCACACCT |
| Suggested primer 2: | GTGTGTGCTACCTCCCACCT |