| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234762 |
| Chromosome: | chromosome 16 |
| Location: | 812377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647602 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTATGATTACGGTATCCCCGTGCGCATT |
| Internal bar code: | AAGTTGCTAGGGGTTAACAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 858 |
| LEAP-Seq percent confirming: | 99.1368 |
| LEAP-Seq n confirming: | 17917 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCATTGCAGTAATTTTCA |
| Suggested primer 2: | GTGTGCTGGACACCATTGAC |