| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.234772 |
| Chromosome: | chromosome 14 |
| Location: | 3274526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629600 | (1 of 1) PF06803 - Protein of unknown function (DUF1232) (DUF1232) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGTTTTGGCCTGCAGTTCCACGACTG |
| Internal bar code: | ATGCAGCGTTGGTTAAACAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 97.3796 |
| LEAP-Seq n confirming: | 1375 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCCACACAGGTTTCG |
| Suggested primer 2: | AGAGGCTCACTGCCATCACT |