| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234776 |
| Chromosome: | chromosome 2 |
| Location: | 5581983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108950 | LF1 | Long Flagella protein 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGCGCAGCCGCTCACCTCTGCCTCTGG |
| Internal bar code: | GTTGGGGCCTGAACCTCTAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 72.2705 |
| LEAP-Seq n confirming: | 748 |
| LEAP-Seq n nonconfirming: | 287 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACAGCACCAGCTTCCA |
| Suggested primer 2: | CTGCATAGGGGTCCTGTTGT |