| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234798 |
| Chromosome: | chromosome 14 |
| Location: | 507358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCAAATTACTACAGGCAGAAGCACA |
| Internal bar code: | CGGTGTTGAGGTCTGGGACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.6148 |
| LEAP-Seq n confirming: | 22242 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGCATGTGCAAATCTAA |
| Suggested primer 2: | ACAACCAGTGATGCCGTACA |