| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234808 |
| Chromosome: | chromosome 1 |
| Location: | 328637 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001950 | Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGCGGGTCAAGCGCGGGACGGCCCACC |
| Internal bar code: | CGGAAAAAGGTTGAGGCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1167 |
| LEAP-Seq percent confirming: | 99.6148 |
| LEAP-Seq n confirming: | 21463 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGTGTGTCAACTGGGAA |
| Suggested primer 2: | CTGGTCATGTTGTGGGAGTG |