Insertion junction: LMJ.RY0402.234817_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.234817
Chromosome:chromosome 1
Location:4087845
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g026800 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATCGAGACGGAGGTGGAGCAGATTCAA
Internal bar code:AGGCTTTTTCAGAGGTGGAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:564
LEAP-Seq percent confirming:26.5625
LEAP-Seq n confirming:102
LEAP-Seq n nonconfirming:282
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCCCTAGTAAGCGTCAA
Suggested primer 2:CATTGATTGTCGCATTGGTC