| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234827 |
| Chromosome: | chromosome_10 |
| Location: | 2277974 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g434400 | PF6 | Flagellar central pair-associated protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGAAATGGCCATCTCTTCCAAGCAAACTCG |
| Internal bar code: | TCTCGCCGGGTGGTGCTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1339 |
| LEAP-Seq percent confirming: | 98.8433 |
| LEAP-Seq n confirming: | 4358 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTTGCTTTAGGTGCAT |
| Suggested primer 2: | GAGCCAGTTCTACGCTGGAG |