| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234830 |
| Chromosome: | chromosome 2 |
| Location: | 5070460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104725 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCGGAGTGAGCCGTGAAACAGCCCACA |
| Internal bar code: | AGGGTAACTGGCGCGGCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1102 |
| LEAP-Seq percent confirming: | 99.4286 |
| LEAP-Seq n confirming: | 4002 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAGTATCAGTGCCGCAG |
| Suggested primer 2: | GGATGGCTATTGCGGACTAC |