| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234835 |
| Chromosome: | chromosome 14 |
| Location: | 585140 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611850 | (1 of 1) K17087 - transmembrane 9 superfamily member 3 (TM9SF3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCTGCGGCGGCGCCTCACGTACTTA |
| Internal bar code: | GATGCATTATGAACGGGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 253 |
| LEAP-Seq percent confirming: | 99.3456 |
| LEAP-Seq n confirming: | 12144 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAAGAAACTTCCAGCG |
| Suggested primer 2: | TGTGTGTGTTCGGTCATCCT |