Insertion junction: LMJ.RY0402.234840_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.234840
Chromosome:chromosome 14
Location:784889
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g613000 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTTGCTGTCCACGAAGCGCGCGTACCG
Internal bar code:CCGTGCAATCTGCGGTATGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:99.5529
LEAP-Seq n confirming:3340
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCATTGATTCATACGCA
Suggested primer 2:CGGTTTAGTTTGGCCTGGTA