| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234890 |
| Chromosome: | chromosome 2 |
| Location: | 3982096 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTTGAGTTGCTGGTCCTGCCAGGCCAA |
| Internal bar code: | GGCTCCAAGTGGCGACGGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 98.7995 |
| LEAP-Seq n confirming: | 6337 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTGTCCCTCATACGTT |
| Suggested primer 2: | TGCCTTTCAAAAGAATTCGG |