Insertion junction: LMJ.RY0402.234895_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.234895
Chromosome:chromosome 3
Location:5386333
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAAAACAGGGGTGCGGGGTGGCCAACTC
Internal bar code:CCACTGGCCCCGCACTGGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:27
LEAP-Seq percent confirming:87.8049
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCGTTGACACCACATATT
Suggested primer 2:GCAGTCGAGCGGTAGGATAG