| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234917 |
| Chromosome: | chromosome 16 |
| Location: | 3579648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681657 | (1 of 1) PF01344//PF13415//PF13418 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGCAGGTGCGCGTGCGTACAGGAGTG |
| Internal bar code: | GTGGGGTTCAATGCGCAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 97.1519 |
| LEAP-Seq n confirming: | 3377 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGATGAACGTGTCTTA |
| Suggested primer 2: | CGTTACCTGTATCGCACCCT |