Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.234983 |
Chromosome: | chromosome_2 |
Location: | 3526263 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095126 | DPE2 | alpha-1,4-glucanotransferase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAACATAGAGCCCCCTGAGTCTCTGCCTG |
Internal bar code: | CTTTCCATATGTTATTTTCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 739 |
LEAP-Seq percent confirming: | 93.5345 |
LEAP-Seq n confirming: | 14944 |
LEAP-Seq n nonconfirming: | 1033 |
LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGCTTCTTCAGGATATGG |
Suggested primer 2: | GCATAGGTGGAGGACGATGT |