| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.234985 |
| Chromosome: | chromosome 5 |
| Location: | 815563 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248100 | ANK1 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCTGCCAGGCCGCCGCCAACCGGGA |
| Internal bar code: | CGGACGGGTATGTTGTCACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 98.6825 |
| LEAP-Seq n confirming: | 18800 |
| LEAP-Seq n nonconfirming: | 251 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCTGTGCTGTCGGTAAC |
| Suggested primer 2: | CTCGGCTCAGTAAACGGAAG |