| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.234993 |
| Chromosome: | chromosome 11 |
| Location: | 2964644 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCGGCCACCACAGCGCCACCGCCGCG |
| Internal bar code: | CCCGCCCGTGATCGCGTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 98.4861 |
| LEAP-Seq n confirming: | 2342 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCTTCCCACACCTTCT |
| Suggested primer 2: | ATGGGAAGGAGAAGGAGGAG |