| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235001 |
| Chromosome: | chromosome 12 |
| Location: | 6070545 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535500 | (1 of 103) IPR000504 - RNA recognition motif domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACCATTGTAATACTGAAGAGGCAAAGT |
| Internal bar code: | GTCGCTCGATTGCGATCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 96.6533 |
| LEAP-Seq n confirming: | 3379 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCTGTGTTTGCTAGGC |
| Suggested primer 2: | AGGGAGTTGGGAAGAAGGAA |