| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235029 |
| Chromosome: | chromosome 2 |
| Location: | 1414123 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGCCAGCCCCGCCTACACACAGCAGG |
| Internal bar code: | AACAGCGCCGCCGCGCCCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 99.0658 |
| LEAP-Seq n confirming: | 4560 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGTAAGGCTCTGGTGAG |
| Suggested primer 2: | GCTTGGATCATGGTTGTGTG |