| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235030 |
| Chromosome: | chromosome 3 |
| Location: | 8209452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCCGTGCCCCATTTCCGGCGCTCTTG |
| Internal bar code: | GCTGCTTGCGCAGAGGAGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 97.7296 |
| LEAP-Seq n confirming: | 2841 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCAACAACAGCACCAG |
| Suggested primer 2: | ACCCGCGATCTGTTTTGTAG |