| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235050 |
| Chromosome: | chromosome 6 |
| Location: | 3749318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278154 | (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCCCAGCGTCCCCTGGTCTAGCCATAG |
| Internal bar code: | ATAGGTGTCCCCACGACCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 99.4388 |
| LEAP-Seq n confirming: | 6733 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCACGTGCTCACTGTAA |
| Suggested primer 2: | GTGGTGTCAGGTGATGAACG |