| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235059 |
| Chromosome: | chromosome 7 |
| Location: | 2200019 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327079 | (1 of 2) IPR002563//IPR012349 - Flavin reductase like domain // FMN-binding split barrel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCGCCTGCTACACTACTGCACCTGCC |
| Internal bar code: | GGGGGGCCAACAGGGGAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 86.3153 |
| LEAP-Seq n confirming: | 5109 |
| LEAP-Seq n nonconfirming: | 810 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAGGTGTGTGTGGAG |
| Suggested primer 2: | GCCTAGCTCCGACAGAATTG |