| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235063 |
| Chromosome: | chromosome 2 |
| Location: | 3560200 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095130 | FAS7,FLA2 | FAS1 domain containing protein;; (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGCGGGACACGGAGCAAGGGGCCTGG |
| Internal bar code: | GTTGAGTCCTTAGTAAATTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.0706 |
| LEAP-Seq n confirming: | 31660 |
| LEAP-Seq n nonconfirming: | 297 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTCTAGAACAGCACCAG |
| Suggested primer 2: | CTGTTGTCGTCTGCTGTCGT |