| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235071 |
| Chromosome: | chromosome 12 |
| Location: | 265345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485418 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGTAGTGCGGGTTGTTGTAGGTCCATG |
| Internal bar code: | CTTCTATTACGCTCTGGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1169 |
| LEAP-Seq percent confirming: | 99.1725 |
| LEAP-Seq n confirming: | 7790 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACATGGAGGAGGCACG |
| Suggested primer 2: | TACTTGTACCCCGTCCGAAG |