| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235091 |
| Chromosome: | chromosome 13 |
| Location: | 2796252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582900 | (1 of 77) IPR001623 - DnaJ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGCCAGTCCTTCAAGACAGGGCTGCGT |
| Internal bar code: | CAGGGTGGATGTGCTTAGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 98.4894 |
| LEAP-Seq n confirming: | 15583 |
| LEAP-Seq n nonconfirming: | 239 |
| LEAP-Seq n unique pos: | 174 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCCCCTGGTTTCTACT |
| Suggested primer 2: | CCACCGCTTGAACTCATTTT |