Insertion junction: LMJ.RY0402.235098_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235098
Chromosome:chromosome 12
Location:8825081
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAAAAACCTGACCCACACCCCCACCCGC
Internal bar code:TGTAGAACACGGTATTAAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:94.4009
LEAP-Seq n confirming:843
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGAATATGGGTGAGGAC
Suggested primer 2:CTGCCCCCACTTCTTAAACA