Insertion junction: LMJ.RY0402.235114_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235114
Chromosome:chromosome 11
Location:2383390
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g474700 BES6 (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCTGGGAGTACCCTAACTAACCTGACG
Internal bar code:GCGTCTGGGGGGGGACGCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:99.3014
LEAP-Seq n confirming:3127
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAGTTCAGCCTTCCTTC
Suggested primer 2:CTTGCAGGTCGGGTATTGTT