Insertion junction: LMJ.RY0402.235118_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.235118
Chromosome:chromosome 16
Location:437529
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g693203 (1 of 5) 3.6.3.27 - Phosphate-transporting ATPase / ABC phosphate transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGTCTGACGCCGTCAGCAGCTCTGTCA
Internal bar code:GGCGCGGTTAGCCTTTCCACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:136
LEAP-Seq percent confirming:99.872
LEAP-Seq n confirming:780
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCAGTCACGCTATCTCGG
Suggested primer 2:ATCTCAGTGTGCGTGTACGG