| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235121 |
| Chromosome: | chromosome 10 |
| Location: | 3737755 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446450 | (1 of 1) PTHR26402:SF316 - TRANSCRIPTIONAL REGULATORY PROTEIN CUSR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCAGGCTGCGCATATCATTGATGGCCC |
| Internal bar code: | CGGGTAAACAGACTGGAAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 98.7547 |
| LEAP-Seq n confirming: | 4996 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTACGGCCCTCAAAGTG |
| Suggested primer 2: | GGAGGAACTCAACTGCTTGC |