| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235127 |
| Chromosome: | chromosome_3 |
| Location: | 2384534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g158850 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGACGCGTTTTTGTCCGTATTTGATACCG |
| Internal bar code: | TAGCCGACCGGGCTTCATATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 98.6182 |
| LEAP-Seq n confirming: | 5567 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGTACAAGGGAAGCGGC |
| Suggested primer 2: | CTTGTCGCCTGCTTATGTGA |