| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235127 |
| Chromosome: | chromosome_3 |
| Location: | 2384537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g158850 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGAACCTGTGTTGCCACCATGCCAAAGGGC |
| Internal bar code: | GGTGACATTGACCAGAGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 94.9109 |
| LEAP-Seq n confirming: | 2928 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGTACAAGGGAAGCGGC |
| Suggested primer 2: | CTTGTCGCCTGCTTATGTGA |