| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235131 |
| Chromosome: | chromosome_6 |
| Location: | 2178668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g265950 | DHC3 | Flagellar inner arm dynein heavy chain, novel minor type dynein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGTTGCATACGCGAAGAAGTCGCAAGGT |
| Internal bar code: | TTACCCGGTGTGACGAAGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1093 |
| LEAP-Seq percent confirming: | 99.2762 |
| LEAP-Seq n confirming: | 15225 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCCATTTCCTACCTGCG |
| Suggested primer 2: | AGAACTTGTCAAACAGGCCC |