| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235143 |
| Chromosome: | chromosome 6 |
| Location: | 6331433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291850 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTTGCCGCATACCGATGGGCCAAAGT |
| Internal bar code: | AGTAAAGAGGGTAAAACGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 97.931 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTCCAGATCGTGTGTG |
| Suggested primer 2: | GAGATGGAGCCAAGAAGTGC |