Insertion junction: LMJ.RY0402.235153_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.235153
Chromosome:chromosome 9
Location:5766724
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g401923 POB6 Proteome of basal body 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGGTCGCCCTCCCTCCGTGTGCGCCAT
Internal bar code:TTTCTGAGTTGCAGTTATCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:434
LEAP-Seq percent confirming:95.1417
LEAP-Seq n confirming:470
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTTGTAGGAGCAGACGG
Suggested primer 2:GCAAAACTTCAGCAAGGAGG