| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235157 |
| Chromosome: | chromosome 3 |
| Location: | 2740745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161800 | MDN1 | (1 of 1) K14572 - midasin (MDN1, REA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTAGTATAACAGTGCAAAGCGCCCGG |
| Internal bar code: | GCCTACGAAATACGCCCGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 99.8227 |
| LEAP-Seq n confirming: | 1126 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTGTGTGTGTGTGTGTG |
| Suggested primer 2: | TTGCCAGGAAAGGTTACGAC |