| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235160 |
| Chromosome: | chromosome 10 |
| Location: | 4581683 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452500 | (1 of 1) PTHR12202//PTHR12202:SF0 - UNCHARACTERIZED // PROTEIN F58B3.4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCGGGAAAGCGAGCCTCCCTCCATGC |
| Internal bar code: | TTGGGAGTCACTGTCCATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 97.9955 |
| LEAP-Seq n confirming: | 6160 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGGTCAACGTGAATG |
| Suggested primer 2: | GTTTCTCCGACATGTTCCGT |