Insertion junction: LMJ.RY0402.235162_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235162
Chromosome:chromosome 12
Location:7936799
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552400 (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGACCTGCCTGTACGTCAGGAAGGTGTT
Internal bar code:CGTGCCGGAGATGGCGGTCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:489
LEAP-Seq percent confirming:99.7409
LEAP-Seq n confirming:385
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTTGAGGTACGACACAT
Suggested primer 2:TCTGCTGTAGCTGACGCACT