Insertion junction: LMJ.RY0402.235164_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.235164
Chromosome:chromosome 11
Location:2031341
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g468400 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGCTTCACATCGTCATTGCACGCTGCA
Internal bar code:CAGGACTCAAGCCACTAGTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:598
LEAP-Seq percent confirming:98.4222
LEAP-Seq n confirming:1310
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTCTGAATGCTTCACACA
Suggested primer 2:CGCTCATCAAACACCTCTCA