| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235171 |
| Chromosome: | chromosome 10 |
| Location: | 676683 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422300 | PRX6 | (1 of 2) K03564 - peroxiredoxin Q/BCP (BCP, PRXQ, DOT5); Peroxiredoxin Q | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGATTCTGAGGGCCGCCGCCGGCAAGCC |
| Internal bar code: | TAATTTTGGCACTTTCGTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.5332 |
| LEAP-Seq n confirming: | 32412 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCCTTGAGGTGTTGCTC |
| Suggested primer 2: | GCTGAGTCCCGAGAAACAAG |