Insertion junction: LMJ.RY0402.235175_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.235175
Chromosome:chromosome 3
Location:9040316
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g210401 (1 of 1) PTHR14614//PTHR14614:SF15 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGACCGCCACACGCCACGCCGCCCCCTG
Internal bar code:GCCCCTGTGGCGATCGCCTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGACAGAGTCGTGGAAACAA
Suggested primer 2:CGATACCCACCATTTTACCG