| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235176 |
| Chromosome: | chromosome 14 |
| Location: | 327802 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610050 | (1 of 1) K15180 - negative elongation factor B (COBRA1, NELFB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATGGGGATGGGGGCGGCAGACGGAGC |
| Internal bar code: | AAATTTGGGGTATGTACAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 99.5536 |
| LEAP-Seq n confirming: | 223 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAACAATGGTACCGAGA |
| Suggested primer 2: | GCCTACAAGTACGAGACCGC |