| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235199 |
| Chromosome: | chromosome 3 |
| Location: | 1281504 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150251 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGCTGTGCACGAGCAGCATGGCTGCT |
| Internal bar code: | CCCTGGCCTCAAAAGCTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 99.244 |
| LEAP-Seq n confirming: | 8533 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCGTGATCTCAACCTCA |
| Suggested primer 2: | TGACAGACAATGCCAAGAGC |