| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235208 |
| Chromosome: | chromosome 16 |
| Location: | 1625679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654250 | THB6 | Truncated hemoglobin; (1 of 2) PTHR10681:SF17 - PEROXIREDOXIN-6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCGTGTGGCCTCGCGGAGTCCAACTGA |
| Internal bar code: | AGGGGACGAGCGCGCGATGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 99.089 |
| LEAP-Seq n confirming: | 52101 |
| LEAP-Seq n nonconfirming: | 479 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGACCTGCTAGGTTGC |
| Suggested primer 2: | TGCGAACACTGAGCATTACC |