| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235217 |
| Chromosome: | chromosome 10 |
| Location: | 2861835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439750 | (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTTGCGCCCCCGACCATGCAAGCCTTG |
| Internal bar code: | TTTTTTCTGACTTTCTGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 98.855 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAGGAGGCGGAAGTAG |
| Suggested primer 2: | ACTCGGTTTCGGTGATTACG |